Fluorol Yellow 088 荧光黄088

货号： JP4473-1G 品牌： Jinpan 标签： 细胞生物学研究

描述

Fluorol Yellow 088 荧光黄088

产品标签

Fluorol Yellow 088 荧光黄088；Solvent Green 4 溶绿4；Suberin lamellae 木栓质层；Ruthenium Red 钌红；Calcofluor White Stain 卡尔科弗卢尔荧光增白剂；CAS NO：81-37-8；

产品信息

产品描述

荧光黄088（Fluorol Yellow 088）是一种多环芳烃有机染料，一种亲脂性荧光素，用于植物组织木栓质层的细胞染色。

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产品特性

保存与运输方法

保存：室温避光干燥保存，2年有效。

运输：室温运输。

应用实例

文献1:Lux A, Morita S, Abe J, Ito K, 2005.An improved method for clearing and staining free-hand sections and whole-mount samples. Ann Bot (Lond) 96:989–996

A）Clearing solution：Lactic acid was saturated with chloral hydrate.

B）(a) A 0.1 % (w/v) solution of berberine hemisulphate (小檗碱) in lactic acid was dissolved at room temperature. (b) A 0.01 % (w/v) solution of fluorol yellow 088 (荧光黄088, JP4473) in lactic acid was prepared by heating at 70°C for 1h. Due to the low stability of the solution, this was prepared fresh before each use.

C) Clearing and staining procedure

The sections floating in drops of clearing solution on microscope slides were heated over a water bath in covered Petri dishes. Treatment for 1h, using a high temperature (70°C), even with large or dense sections, was usually sufficient. The clearing solution was then absorbed by pipette or tissue paper and the sections were thoroughly washed with distilled water added several times to the sections and absorbed by tissue paper. Sections were post-stained and subsequently washed in the same way. Framing by a liquid blocker (PAP pen) proved useful to prevent the loss of sections from slides.

For observation of cell files along the root axis,whole roots were cleared and stained in lactic acid with fluorescence stains (berberine or fluorol yellow) and post-stained with safranin O.This procedure allows observation of epidermal and exodermal cells in thick roots of various species, and in thin roots of arabidopsis it works well for staining and observation of endodermal cells. In thick roots such as those of maize or sorghum, peeling peripheral root tissues exposes the endodermal cells and allows their direct observation. The contrast of cell walls is increased after treatment of peeled samples with berberine in lactic acid.

图1.「D-H」甜瓜不定根的切片经透明处理和含荧光黄088的乳酸染色液染色，在白光「D」、紫外光「E」和两种光下的重叠图片「F」。

文献2: Cohen H et al. A Multilevel Study of Melon Fruit Reticulation Provides Insight into Skin Ligno-Suberization Hallmarks. Plant Physiol. 2019 Apr;179(4):1486-1501.

A）Histochemical observations of suberin and lignin in skin cross sections were achieved by staining with a freshly prepared solution of Fluorol Yellow 088 (0.01% [w/v] in lactic acid) for 30 min at 70°C and phloroglucinol-hydrochloric acid (2% [w/v] in 50% hydrochloric acid) for 30 min at room temperature, respectively. Stained sections were then observed with an Olympus CLSM500 microscope using bright-field and GFP filters. Autofluorescence of cuticle layers in smooth skin sections was observed with a Cy5 filter.

图2.光滑和网状果皮横截面切片的木质素（lignin）（间苯三酚-盐酸染色）和木栓质（suberin）（荧光黄088）染色。

文献3: Gunawardena AH et al. Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae). Am J Bot. 2007 Jul;94(7):1116-28.

A）For fluorol yellow 088, a final concentration of 0.01% (w/v) was made by dissolving 0.01 g of fluorol yellow 088 in 50 mL of PEG 400 solution and heating at 90°C for 1 h (Brundrett et al., 1991). An equal volume of 90% (v/v) glycerol solution was added to the PEG staining solution. Leaves were stained for 1 h at room temperature, rinsed several times with distilled water, and observed with UV light for a bright yellow fluorescence. Unstained cell walls were also examined for autofluorescence as a test for the phenolic constituents of suberin.

图3：成熟花边植物叶的细胞壁组织化学检测。荧光黄088染脂肪族物质「A-D」。透化叶子（A，B）；新鲜叶子（C，D）。微分干涉相衬显微成像图片观察到棕色色素，而荧光成像图片观察到阳性染色（黄绿色荧光）。

注意事项

1）本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

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