人Osteoprotegerin/TNFRSF11B ELISA试剂盒96T


人Osteoprotegerin/TNFRSF11B ELISA试剂盒

简要描述:人Osteoprotegerin/TNFRSF11B ELISA试剂盒,产品型号:96T。
灵敏度:6.8 pg/mL
检测范围:62.5 – 4,000 pg/mL
回收率范围:85.0-108.9%
保存方法:2-8℃

详细介绍

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品牌 其他品牌 供货周期 一个月
应用领域 医疗卫生,环保,化工,生物产业,综合

人Osteoprotegerin/TNFRSF11B ELISA试剂盒产品型号:96T。

人Osteoprotegerin/TNFRSF11B ELISA试剂盒,灵敏度:6.8 pg/mL,检测范围:62.5 – 4,000 pg/mL,回收率范围:85.0-108.9%,保存方法:2-8℃。

产品名称:Human Osteoprotegerin/TNFRSF11B ELISA Kit

描述:

检测原理:

本实验采用双抗体夹心 ELISA 法。抗人OPG单抗包被于微孔板上,样品和标准品中的OPG会与固定在板上的抗体结合,游离的成分被洗去;加入生物素标记的抗人OPG多抗,与结合在微孔板上的OPG结合,游离的成分被洗去;再加入特异性识别生物素的链霉亲和素标记的辣根过氧化物酶,形成复合物,洗去游离成分后,加入底物溶液(显色剂),溶液颜色逐渐变成蓝色,加入终止液溶液变黄并且停止变化。用酶标仪测定吸光度。

检测类型:双抗夹心法

形式:预包被96孔板

检测样本类型:细胞上清液,血清,血浆

上样量:100 μl


上海金畔生物科技有限公司

  1. 国内试剂耗材经销代理。

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OPG/ TNFRSF11B (C-6His), Human, Recombinant


OPG/ TNFRSF11B (C-6His), Human, Recombinant

货号:PCK213

价格:¥600¥1480¥8880¥12880

规格:

10µg

  • 10µg
  • 50µg
  • 500µg
  • 1mg

  • 产品概述
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产品概述

产品信息

别名 Tumor Necrosis Factor Receptor Superfamily Member 11B; Osteoclastogenesis Inhibitory Factor; Osteoprotegerin; TNFRSF11B; OCIF; OPG
物种 Human
表达宿主 Human Cells
序列信息 asd
检索号 O00300
分子量 44.65 kDa
表观分子量 57 kDa
生物活性 Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAILThe ED50 for this effect is less than 25 ng/ ml.

产品特性

纯度 >95% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU per µg as determined by LAL test.
保存 Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

TNFRSF11B is a secreted Protein, containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy Receptor for the Receptor activator of nuclear factor kappa B Ligand (RANKL). By binding RANKL, TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/ macrophages and are Derived from granulocyte/ macrophage-forming colony units (CFU-GM)) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/ stromal cells, blocks the RANKL-RANK Ligand interaction between osteoblast/ stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.
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