(1S,3R)-RSL3 (GPx4 inhibitor) 谷胱甘肽过氧化物酶 4抑制剂 货号: MZ5273-100MG 品牌: Jinpan 标签: 信号转导研究
描述
(1S,3R)-RSL3 (GPx4 inhibitor)
谷胱甘肽过氧化物酶 4抑制剂
产品关键词:
(1S,3R)-RSL3;GPx4 inhibitor;谷胱甘肽过氧化物酶4抑制剂;Ferroptosis activator;铁死亡激活剂;C11 BODIPY 581/591;CAS:1219810-16-8;
产品信息
产品名称
|
产品编号 |
CAS NO. |
规格 |
价格(元) |
(1S,3R)-RSL3 (GPx4 inhibitor)
|
MZ5273-10MG |
1219810-16-8 |
10mg |
453
|
(1S,3R)-RSL3 (GPx4 inhibitor) |
MZ5273-50MG |
1219810-16-8 |
50mg |
1323
|
(1S,3R)-RSL3 (GPx4 inhibitor) |
MZ5273-100MG |
1219810-16-8 |
100mg |
2123
|
产品描述
(1S,3R)-RSL3是一种谷胱甘肽过氧化物酶 4 (GPX4) 抑制剂,诱导致命性的脂质氢过氧化物累积和铁死亡。与野生型BJeHLT细胞(EC50 = 2 µM)相比,选择性作用于表达HRASV12的BJeHLT细胞(EC50 = 0.01 µM)。各种小鼠移植瘤模型中,(1S,3R)-RSL3减少肿瘤体积,其中带弥漫大B细胞淋巴瘤和肾细胞癌的模型最敏感。
产品特性
化学名:(1S,3R)-Methyl 2-(2-chloroacetyl)-2,3,4,9-tetrahydro-1-[4-(methoxycarbonyl)phenyl]-1H-pyrido[3,4-b] indole-3-carboxylate |
CAS NO:1219810-16-8 |
同义名:RSL3 |
分子式:C23H21ClN2O5 |
分子量:440.88 |
外观:固体 |
纯度:≥98% |
溶解性:溶于DJPO(30mg/ml)、乙醇(25mg/ml)、DMF(30mg/ml) |
化学结构式: |
保存与运输方法
保存:-20℃避光干燥保存,至少2年有效。
运输:冰袋运输。
注意事项
1)针对溶解性比较差的化合物,可通过37℃孵育以及超声的方式来促进其溶解。
2)本品并非商业化的临床药物,仅用作科研用途,不得用作临床诊断或治疗,不得用于食品或药品,绝对禁止用在人身上。
3)为了您的安全和健康,请穿实验服并戴一次性手套操作。
储存液制备
质量
溶剂体积
浓度 |
1mg |
5mg |
10mg |
1mM |
2.2682 mL |
11.3410 mL |
22.6819 mL |
5mM |
0.4536 mL |
2.2682 mL |
4.5364 mL |
10mM |
0.2268 mL |
1.1341 mL |
2.2682 mL |
【温馨提示】:请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。储备液置于-80°C保存,约6个月有效;-20°C保存,约1个月有效。溶液储存的过程中避免强光直射。
使用方法【源自文献,仅作参考】
文献1,Zhang, W.; Li, X.; Xu, J.; Wang, Y.; Xing, Z.; Hu, S.; Fan, Q.; Lu, S.; Cheng, J.; Gu, J.; Cai, R. The RSL3 Induction of KLK Lung Adenocarcinoma Cell Ferroptosis by Inhibition of USP11 Activity and the NRF2-GSH Axis. Cancers 2022, 14, 5233. https://doi.org/10.3390/cancers14215233
体外研究(细胞水平):
细胞类型(Cell Type):A549 and H2122 cells
实验方法(Assay):A549 cells were treated with dimethyl sulfoxide (DJPO) or RSL3 (4 μM) for 12 h and H2122 cells were treated with DJPO or RSL3 (8 μM) for 12 h and then subjected to CCK-8 assay.
体内研究(动物模型):动物模型(Animal Model):10-week-old male nude mice were subcutaneously injected with A549 cells (100 μL containing 5 × 106 cells/injection) and monitored for tumor cell xenografts to reach approximately 100 mm3. 实验方法(Assay):The mice were then divided into two groups (n = 5), the RSL3 treatment (100 mg/kg; dissolved in 5% dimethyl sulfoxide/corn oil; administrated intratumorally twice a day for one week) and control (5% dimethyl sulfoxide/corn oil only) groups. After that, the mice were sacrificed, and tumor weight was measured respectively.
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其他信息
品牌: |
Jinpan
|
CAS: |
1219810-16-8
|
规格: |
100mg
|
货期: |
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