体内研究（Lectin Perfusion）：

动物模型（Animal Model）：Xenografts were established in 4- to 6-week-old female NCR nude mice by intrarenal injection of 106 SK-NEP-1 cultured human Wilms tumor cells and allowed to grow. After 5 weeks, large tumors were palpable in all mice, and a cohort was randomly selected to provide day-0 controls.

灌注方法（Lectin perfusion）：Selected mice at each time point underwent intravascular injection of fluorescein- labeled Lycopersicon esculentum lectin (100 μg in 100 μl of saline) into the left ventricle. The vasculature was fixed by infusion of 1% paraformaldehyde (pH 7.4) in PBS and then washed by perfusion of PBS.

给药途径（Administration）：Intravascular (i.v.) injection

体内研究（Lectin Perfusion）：

动物模型（Animal Model）：Tumor-bearing RIP-Tag2 transgenic mice34 (C57BL/6 background, 10 to 12 weeks of age).

灌注方法（Lectin perfusion）：In mice used for immunohistochemistry, blood flow and patency of individual tumor vessels were assessed by injection of 100 μg of fluorescein isothiocyanate-labeled Lycopersicon esculentum lectin in 0.9% NaCl (100 μl into femoral vein). Two minutes later the chest was opened rapidly, and the vasculature was perfused for 2 minutes at a pressure of 120 mmHg with fixative [1% paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4] from an 18-gauge cannula inserted into the aorta via an incision in the left ventricle. Blood and fixative exited through an opening in the right atrium. Tissues were removed, immersed in fixative for 1 hour at 4°C, and then processed for immunohistochemistry.

给药途径（Administration）：Intravascular (i.v.) injection

体外研究：

细胞类型（Cell type）：Microglia and endothelial cells

实验方法（Assay）：Take 25 μl of 2mg/ml Lycopersicon esculentum (tomato) lectin FITC conjugate stock solution into 2.5ml r-ACSF to give 20 μg/ml concentration. Pipette the dye over the slices as before, leaving the slices incubating for a period of 45 minutes.

Jinpan

N/A

500μg

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