同仁化学Hampton蛋白结晶试剂盒Ammonium formate/HR2-659| 日本DOJINDO

发表于2024年2月28日由上海金畔生物科技有限公司

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Products > Optimize Reagents > Optimize – Salts > Ammonium formate

Ammonium formate

CAT NO

HR2-659

NAME

10.0 M Ammonium formate

DESCRIPTION

200 mL

PRICE

$192.00

支持材料

HR2-659 Ammonium formate SDS

应用

Crystallization grade Ammonium formate for formulating screens or for optimization
结晶级甲酸铵，用于筛选或优化

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium formate

Synonyms: Formic acid ammonium salt
HCOONH₄
CH₅NO₂
M_r 63.06
CAS 540-69-2
EC Number 208-753-9
Beilstein Registry Number 3625095
Merck 14,523
RTECS BQ6650000
MDL Number MFCD00013103
PubChem Substance ID 24846279
Purity ≥ 99.0%

Measured pH Range: 7.0 – 7.3 at 25°C
Measured Conductivity Range: 515.6 – 725.2 mS/cm at 25°C
Measured Refractive Index Range: 1.38437 – 1.40423 at 20°C

别名：甲酸铵盐
HCOONH4
CH5NO2
63.06 先生
CAS 540-69-2
欧盟编号 208-753-9
贝尔斯坦注册号 3625095
默克 14,523
RTECS BQ6650000
MDL 编号 MFCD00013103
PubChem 物质 ID 24846279
纯度≥99.0%

测得的 pH 值范围：25°C 时 7.0 – 7.3
测得的电导率范围：25°C 时 515.6 – 725.2 mS/cm
测得的折射率范围：20°C 时为 1.38437 – 1.40423

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

参考Ammonium formate

1. Picornaviruses of two different genera have similar structures. Luo, M; Arnold, E; Erickson, JW; Rossmann, MG; Boege, U; Scraba, DG. J Mol Biol 180, 703- 714, 1984.

2. The crystal structure of the Venezuelan equine encephalitis alphavirus nsP2 protease. Andrew T. Russo, Mark A. White, and Stanley J. Watowich. Structure 14, 1449-1458, September 2006.

1. 两个不同属的小核糖核酸病毒具有相似的结构。罗，男；阿诺德，E；埃里克森，JW；罗斯曼，MG；博格，U；斯克拉巴，DG。分子生物学杂志 180, 703-714, 1984。

2.委内瑞拉马脑炎甲病毒nsP2蛋白酶的晶体结构。 Andrew T. Russo、Mark A. White 和 Stanley J. Watowich。结构 14，1449-1458，2006 年 9 月。

同仁化学Hampton蛋白结晶试剂盒Dialysis Buttons/HR3-336/HR3-316/HR3-332/HR3-330| 日本DOJINDO

发表于2024年2月28日由上海金畔生物科技有限公司

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理中国代理商

Products > Crystallization Plates, Hardware & Accessories > Dialysis Buttons, Membranes & Applicators > Dialysis Buttons

Dialysis Buttons

应用

Crystallization by dialysis, protein folding, & small volume sample dialysis
通过透析、蛋白质折叠和小体积样品透析进行结晶

特征

Low volume dialysis
Fits in 24 well plates
Use over and over again
低容量透析
适合 24 孔板
反复使用

描述

In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.^1-3

Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

在透析方法中，有问题的样品通过半透膜与“沉淀剂”分离，半透膜允许小试剂分子通过，但阻止生物大分子穿过膜。1-3

透析按钮由聚苯乙烯注塑成型，大小与小按钮相当。将样品放置在该腔室中，以便在按钮顶部形成一个轻微的液体圆顶。透析膜放置在按钮/样品的顶部，并用 O 形环固定到位。 O 形环由透析按钮中的凹槽固定到位。透析按钮配有 O 形圈。 5 µl 至 100 µl 大小的透析按钮的总高度约为 6 mm，外径约为 10 mm，未安装 O 形圈，直径约为 12 mm，安装了 O 形圈。 200 µl 和 350 µl 大小的透析按钮总高度约为 8 mm，外径约为 15 mm，不带 O 形圈，直径约为 17 mm，带 O 形圈。每个透析按钮包都包括一个塑料高尔夫球 T 形涂抹器，以帮助将透析膜应用到按钮上并使用随附的 O 形圈固定。

HR3-336 Dialysis Buttons Sampler

Hampton蛋白结晶试剂盒Dialysis Buttons/HR3-336/HR3-316/HR3-332/HR3-330

$105.00

支持材料

Dialysis Button User Guide

参考

1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).

2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).

3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.

4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.

1. McPherson, A.，蛋白质晶体的制备和分析，Krieger 出版社，88-91 (1992)。

2. Durcruix, A 和 Giege, R.，核酸和蛋白质的结晶，实用方法，牛津大学出版社，78-82 (1992)。

3. Zeppenzauer, M. 等人，Acta Chem Scan (1967) 21, 1009。

4. 呼吸复合体膜结构域的结构 I. Efremov RG, Sazanov LA。自然。 2011 年 8 月 7 日；476(7361):414-20。 doi：10.1038/nature10330。

同仁化学Hampton蛋白结晶试剂盒Dialysis Membrane Discs for Buttons/HR3-344/HR3-338/HR3-346| 日本DOJINDO

发表于2024年2月27日由上海金畔生物科技有限公司

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理中国代理商

Products > Crystallization Plates, Hardware & Accessories > Dialysis Buttons, Membranes & Applicators > Dialysis Membrane Discs for Buttons

Dialysis Membrane Discs for Buttons

应用

Membranes for dialysis
透析膜

描述HR3-338 Dialysis Membrane Discs MW Cutoff 3500

Spectra/Por® regenerated cellulose dialysis membrane discs are 33 mm, circular, pre-cut membranes available with the following molecular weight cutoffs:
• 3,500 MW cutoff
• 6,000 – 8,000 MW cutoff
• 12,000 – 14,000 MW cutoff

Spectra/Por® 再生纤维素透析膜片是 33 毫米的圆形预切膜，具有以下截留分子量：
• 3,500 兆瓦截止
• 6,000 – 8,000 MW 截止
• 12,000 – 14,000 兆瓦截止

Hampton蛋白结晶试剂盒Dialysis Membrane Discs for Buttons/HR3-344/HR3-338/HR3-346

$321.00

同仁化学Hampton蛋白结晶试剂盒Ammonium phosphate dibasic/HR2-629| 日本DOJINDO

发表于2024年2月26日由上海金畔生物科技有限公司

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理中国代理商

Products > Optimize Reagents > Optimize – Salts > Ammonium phosphate dibasic

Ammonium phosphate dibasic

CAT NO

HR2-629

NAME

3.5 M Ammonium phosphate dibasic

DESCRIPTION

200 mL

PRICE

$104.00

应用

Crystallization grade Ammonium phosphate dibasic for formulating screens or for optimization
结晶级磷酸氢二铵，用于筛选或优化

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium phosphate dibasic

Synonyms: Ammonium hydrogenphosphate or di-Ammonium hydrogen phosphate (sec) or Diammonium hydrogen phosphate
(NH₄)₂HPO₄
H₉N₂O₄P
M_r 132.06
CAS Number [7783-28-0]
EC Number 231-987-8
Merck 14,542
MDL Number MFCD00010891
PubChem Substance ID 24846462
Purity ≥ 99.0%

Measured pH range: 7.8 – 8.2 at 25°C
Measured Conductivity Range: 87.4 – 106.8 mS/cm at 25°C
Measured Refractive Index Range: 1.40307 – 1.40420 at 20°C

别名：磷酸氢铵或磷酸氢二铵 (sec) 或磷酸氢二铵
(NH4)2HPO4
H9N2O4P
132.06 先生
CAS 编号 [7783-28-0]
欧盟编号 231-987-8
默克 14,542
MDL 编号 MFCD00010891
PubChem 物质 ID 24846462
纯度≥99.0%

测得的 pH 值范围：25°C 时为 7.8 – 8.2
测得的电导率范围：25°C 时 87.4 – 106.8 mS/cm
测得的折射率范围：20°C 时为 1.40307 – 1.40420

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
NO₃ ≤0.001%
Pb ≤0.0005%
SO₄ ≤0.002%
Sr ≤0.0005%
Zn ≤0.0005%

支持材料Ammonium phosphate dibasic

HR2-629 Ammonium phosphate dibasic SDS

同仁化学Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312| 日本DOJINDO

发表于2024年2月26日由上海金畔生物科技有限公司

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理中国代理商

Products > Crystallization Plates, Hardware & Accessories > Micro-Bridges > Micro-Bridges

Micro-Bridges

应用

Sitting drop crystallization
Heavy atom soaks
Seeding
坐滴结晶
重原子浸泡
播种

特征

Perform sitting drop experiments in 24 well hanging drop plates
Removable
在 24 孔悬挂滴板中进行坐滴实验
可拆卸的

描述

Micro-Bridges are small devices in the shape of a bridge that are designed to carry out sitting drop vapor diffusion crystallization when placed in a VDX™ Plate or Linbro® Plate. A single Micro-Bridge fits neatly into the reservoir of a standard 24 well VDX Plate for a sitting drop crystallization experiment. Once placed inside the wells, Micro-Bridges are stable and there is no need to stick them to the wells with grease or adhesive. It is therefore possible to transfer them to other wells during or after a crystallization experiment.

Why sitting drop? Placing the droplet in the indentation greatly reduces the risk of losing the protein by accident. Crystallization can be carried out in the presence of detergents and organic solvents which are compatible with polystyrene (such as MPD, iso-propanol, and ethanol). The protein drop is less affected by condensation problems. Soaking and seeding experiments can be carried out easily. Crystals can be transported more securely. Larger drop volumes can be used. Micro-Bridges have a concave indentation in the top surface of the bridge which holds the sample droplet during a crystallization experiment and prevents the droplet from spreading over a large area. Made from polystyrene, these parts are highly transparent and suitable for most crystallizations. The surface of the indentation is highly polished to facilitate the visual inspection of the drops under a microscope. The maximum drop volume for the Micro-Bridge is 35 µl. Reservoirs can be sealed with plain cover slides and vacuum grease or clear sealing tape.

Approximate dimensions: Height: 12 mm Length: 14 mm Width: 7 mm

微桥是桥形的小型设备，设计用于在放置在 VDX™ 板或 Linbro® 板中时进行坐滴蒸汽扩散结晶。单个 Micro-Bridge 可巧妙地装入标准 24 孔 VDX 板的储液器中，用于坐滴结晶实验。一旦放置在孔内，Micro-Bridges 就很稳定，无需用油脂或粘合剂将它们粘在孔上。因此，可以在结晶实验期间或之后将它们转移到其他孔中。

为什么要坐下来？将液滴放入压痕中可大大降低意外丢失蛋白质的风险。可以在与聚苯乙烯相容的去污剂和有机溶剂（如 MPD、异丙醇和乙醇）存在下进行结晶。蛋白质下降受冷凝问题的影响较小。可以轻松进行浸泡和播种实验。水晶可以更安全地运输。可以使用更大的液滴体积。微桥在桥的顶部表面有一个凹形压痕，用于在结晶实验期间固定样品液滴并防止液滴在大面积上扩散。这些部件由聚苯乙烯制成，高度透明，适用于大多数结晶。压痕表面经过高度抛光，便于在显微镜下目视检查液滴。 Micro-Bridge 的最大滴体积为 35 µl。可以使用普通盖玻片和真空润滑脂或透明密封胶带密封储液罐。

大概尺寸：高度：12 毫米长度：14 毫米宽度：7 毫米

HR3-310/HR3-312 Micro-Bridges

Hampton蛋白结晶试剂盒Micro-Bridges/HR3-310/HR3-312

CAT NO

HR3-310

NAME

Micro-Bridges

DESCRIPTION

100 pack

PRICE

$86.00

CAT NO

HR3-312

NAME

Micro-Bridges

DESCRIPTION

400 pack

PRICE

$297.00

支持材料

HR3-310 HR3-312 Micro-Bridges User Guide

参考

1. Structure of aluminum-bound ovotransferrin at 2.15 angstrm resolution. K, Mizutani, B. Mikami, S. Aibara, and M. Hirose. Acta Cryst (2005) D61, 1636-1642.

1. 2.15 埃分辨率下铝结合卵转铁蛋白的结构。 K、Mizutani、B. Mikami、S. Aibara 和 M. Hirose。水晶学报 (2005) D61, 1636-1642。

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Ammonium formate

CAT NO

NAME

DESCRIPTION

PRICE

支持材料

应用

特征

描述Ammonium formate

参考Ammonium formate

Dialysis Buttons

应用

特征

描述

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CAT NO

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相关项目

参考

Dialysis Membrane Discs for Buttons

应用

描述HR3-338 Dialysis Membrane Discs MW Cutoff 3500

CAT NO

NAME

DESCRIPTION

PRICE

CAT NO

NAME

DESCRIPTION

PRICE

CAT NO

NAME

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相关项目

Ammonium phosphate dibasic

CAT NO

NAME

DESCRIPTION

PRICE

应用

特征

描述Ammonium phosphate dibasic

支持材料Ammonium phosphate dibasic

Micro-Bridges

应用