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同仁化学Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725| 日本DOJINDO

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上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

  • Crystallization grade Tris for formulating screens or for optimization
  • 结晶级 Tris 用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词： Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围：7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围：25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围：25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率：20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围：25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率：20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-589 Tris SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-725 Tris pH 8.5 SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 Titration Table

相关项目

  • Hydrochloric acid
  • StockOptions Tris Buffer Kit
  • Individual StockOptions Tris Reagents
  • 盐酸
    StockOptions Tris 缓冲套件
    个人股票期权 Tris 试剂

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。 田，Y； 比曼，台湾； 罗德里克，SL； 蛋白质 28, 298-300, 1997。

同仁化学Hampton蛋白结晶试剂盒NDSB-195/HR2-703| 日本DOJINDO

发表于由

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-195

NDSB-195

Hampton蛋白结晶试剂盒NDSB-195/HR2-703

CAT NO

HR2-703

NAME

NDSB-195

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

Hampton蛋白结晶试剂盒NDSB-195/HR2-703 HR2-703 NDSB-195 User GuideHampton蛋白结晶试剂盒NDSB-195/HR2-703 HR2-703 NDSB-195 SDS

应用

  • Crystallization grade NDSB-195 for formulating screens or for optimization
  • 结晶级 NDSB-195 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-195
Synonyms: Dimethylethylammonium propane sulfonate or Non-detergent sulfobetaine
C7H17NO3S
Mr 195.30
Purity: ≥95% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

非去污剂磺基甜菜碱在很宽的 pH 值范围内是两性离子的，很容易通过透析去除，并且在近紫外范围内不会显着吸收。

蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。

NDSB-195
别名：二甲基乙基丙烷磺酸铵或非洗涤剂磺基甜菜碱
C7H17NO3S
195.30 先生
纯度：TLC ≥95%
吸湿性
常温（+20℃）保存

HR2-703

参考

1. Goldberg, M.E., et al. 1995. Folding & Design 1, 21.

2. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

3. Vuillard, L., et al. 1995. Biochem. J. 305, 337.

4. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

同仁化学Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727| 日本DOJINDO

发表于由

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > TRIS hydrochloride Buffer

TRIS hydrochloride Buffer

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727

CAT NO

HR2-579

NAME

1.0 M TRIS hydrochloride

DESCRIPTION

100 mL

PRICE

$63.00

CAT NO

HR2-727

NAME

1.0 M TRIS hydrochloride pH 8.5

DESCRIPTION

100 mL

PRICE

$63.00

支持材料

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 HR2-579 TRIS hydrochloride SDSHampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 HR2-727 TRIS hydrochloride pH 8.5 SDSHampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 Titration Table

应用

  • Crystallization grade TRIS hydrochloride buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 TRIS 盐酸盐缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述TRIS hydrochloride

Synonyms: TRIS HCl or Trizma hydrochloride or Tris(hydroxymethyl)aminomethane hydrochloride
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
Mr 157.60
[1185-53-1]
EC Number 214-684-5
Beilstein Registry Number 3675235
MDL Number MFCD00012590
PubChem Substance ID 24900398
Purity ≥ 99.0%

Titrate HR2-579 to the desired pH using NaOH
HR2-579 Measured pH Range: 3.9 – 4.0 at 25°C
HR2-579 Measured Conductivity Range: 52.3 – 53.7 mS/cm at 25°C
HR2-579 Measured Refractive Index Range: 1.35840 – 1.35891 at 20°C

HR2-727 has been titrated to pH 8.5 using NaOH
HR2-727 Measured Conductivity Range: 59.8 – 61.7 mS at 25°C
HR2-727 Measured Refractive Index Range: 1.35938 – 1.36019 at 20°C

Use StockOptions Tris Hydrochloride kit (HR2-237) for a set of 21 Tris hydrochloride reagents titrated to pH 7.0 to 9.0 in 0.1 pH increments.

同义词：TRIS HCl 或 Trizma 盐酸盐或 Tris(羟甲基)氨基甲烷盐酸盐
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
157.60 先生
[1185-53-1]
欧盟编号 214-684-5
贝尔斯坦注册号 3675235
MDL 编号 MFCD00012590
PubChem 物质 ID 24900398
纯度≥99.0%

使用 NaOH 将 HR2-579 滴定至所需的 pH 值
HR2-579 测量的 pH 范围：25°C 时 3.9 – 4.0
HR2-579 测量的电导率范围：25°C 时为 52.3 – 53.7 mS/cm
HR2-579 测量的折射率范围：20°C 时为 1.35840 – 1.35891

HR2-727 已使用 NaOH 滴定至 pH 8.5
HR2-727 测得的电导率范围：25°C 时为 59.8 – 61.7 mS
HR2-727 测量的折射率范围：20°C 时为 1.35938 – 1.36019

将 StockOptions Tris 盐酸试剂盒 (HR2-237) 用于一套 21 种 Tris 盐酸试剂，以 0.1 的 pH 增量滴定至 pH 7.0 至 9.0。

Al  ≤0.0005%
As ≤0.0001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%

TRIS hydrchoride

相关项目

  • Sodium hydroxide
  • StockOptions Tris Hydrochloride Buffer Kit
  • Individual StockOptions Tris Hydrochloride Reagents
  • 氢氧化钠
    StockOptions Tris 盐酸缓冲液试剂盒
    个别股票期权 Tris 盐酸盐试剂

同仁化学Hampton蛋白结晶试剂盒NDSB-201/HR2-701| 日本DOJINDO

发表于由

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201

NDSB-201

Hampton蛋白结晶试剂盒NDSB-201/HR2-701

CAT NO

HR2-701

NAME

NDSB-201

DESCRIPTION

5 grams

PRICE

$43.00

支持材料

Hampton蛋白结晶试剂盒NDSB-201/HR2-701 HR2-701 NDSB-201 User GuideHampton蛋白结晶试剂盒NDSB-201/HR2-701 HR2-701 NDSB-201 SDS

应用

  • Crystallization grade NDSB-201 for formulating screens or for optimization
  • 结晶级 NDSB-201 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C8H11NO3S
Mr 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).

3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.

同仁化学Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581| 日本DOJINDO

发表于由

上海金畔社生物科技有限公司日本同仁化学dojindo全线产品代理 中国代理商

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Hydrochloric acid

Hydrochloric acid

Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581

CAT NO

HR2-581

NAME

1.0 M Hydrochloric acid

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581 HR2-581 Hydrochloric acid SDS

应用

  • Crystallization grade Hydrochloric acid for formulating screens or for optimization
  • 结晶级盐酸，用于筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Hydrochloric acid

Synonyms: None
HCl
Mr 36.46
CAS Number [7647-01-0]
EC Number 231-595-7
Beilstein Registry Number 1098214
Merck 14,4794
RTECS MW4025000
MDL Number MFCD00011324
PubChem Substance ID 329754537
UN Number (RIDADR) UN 1789 8/PG 2

Measured Conductivity Range: 320.4 – 378.2 mS/cm at 25°C

The 1.0 M Hydrochloric acid is formulated using pyrogen free (<0.03 Endotoxin (EU/ml)) water. The final 1.0 M Hydrochloric acid is not certified pyrogen free.

同义词：无
盐酸
36.46 先生
CAS 编号 [7647-01-0]
欧盟编号 231-595-7
贝尔斯坦注册号 1098214
默克 14,4794
RTECS MW4025000
MDL 编号 MFCD00011324
PubChem 物质 ID 329754537
联合国编号 (RIDADR) UN 1789 8/PG 2

测得的电导率范围：25°C 时 320.4 – 378.2 mS/cm

使用无热原 (<0.03 内毒素 (EU/ml)) 的水配制 1.0 M 盐酸。 最后的 1.0 M 盐酸未经过认证无热原。

Ag ≤0.000001%
Al  ≤0.000005%
As ≤0.0000005%
Ba ≤0.000002%
Bi  ≤0.00001%
Br  ≤0.005%
Ca ≤0.00005%
Cd ≤0.000001%
Co ≤0.000001%
Cr ≤0.000002%
Cu ≤0.000002%
Fe ≤0.00002%
Hg ≤0.00001%
K   ≤0.00001%
Li   ≤0.000001%
Mg ≤0.00001%
Mn ≤0.000001%
Mo ≤0.000002%
Na ≤0.00005%
NH4+ ≤0.0001%
Ni   ≤0.000002%
PO4 ≤0.0005%
Pb   ≤0.000002%
SO4 ≤0.0001%
SO3 ≤0.0001%
Sr   ≤0.000001%
TI   ≤0.000005%
Zn   ≤0.000005%

Hydrochloric acid相关项目

  • BIS-TRIS Buffer
  • HEPES sodium Buffer
  • Imidazole Buffer
  • Sodium acetate trihydrate Buffer
  • Sodium cacodylate trihydrate Buffer
  • Sodium citrate tribasic dihydrate Buffer
  • Tris Buffer
  • BIS-TRIS 缓冲器
    HEPES钠缓冲液
    咪唑缓冲液
    三水乙酸钠缓冲液
    二甲胂酸钠三水合物缓冲液
    柠檬酸钠二水合物缓冲液
    Tris 缓冲区