SD/-Ura Broth 酵母缺陷型培养基(肉汤)
货号: JP6367-5L 品牌: Jinpan 标签: 生化试剂
描述
SD/-Ura Broth
产品标签
YPD Medium(YEPD Medium);YPDA Medium;YNB; Minimal SD Base;Dropout (DO) Supplements 氨基酸缺失混合物;
产品信息
产品名称 |
产品编号 |
规格 |
价格(元) |
SD/-Ura Broth 酵母缺陷型培养基(肉汤) |
JP6367-5L |
5L |
958 |
产品描述
酵母缺陷型培养基是由SD Base + DO Supplement,以添加琼脂和未添加琼脂两种方式混合而成的完全培养基,前者是固体培养基,后者是液体培养基(肉汤),用于选择培养含特异载体的酵母或进行杂交筛选。
Minimal SD Base,即Minimal Synthetical Defined Medium,是在无氨基酸酵母氮源基础(YNB)上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。Dropout (DO) Supplement,即氨基酸缺陷混合物(或省却成分添加剂)是缺乏一种或多种特定氨基酸的混合物。
SD/-Ura Broth就是缺乏尿嘧啶(Ura)的酵母缺陷型液体培养基,主要用于单质粒酵母转化筛选和单/双杂交系统报告基因检测。
保存与运输方法
保存:室温干燥保存,至少2年稳定。
运输:室温运输。
培养基配制
1) 称取28g粉末加入1L去离子水,搅拌混匀使其溶解。
2) 重要:测试培养基pH是否为5.8,若有必要用弱酸或弱碱调整pH。
3) 121℃高压灭菌15min。4℃避光保存已灭菌的液体培养基。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。
附表1 酵母培养基配制浓度表
培养基类型 |
名称 |
货号 |
终浓度(g/L) |
Rich Media
富集培养基 |
YPD Agar Medium |
JP6301-350G |
70 |
YPD Medium |
JP6302-250G |
50 |
YPDA Agar Medium |
JP6303-350G |
70 |
YPDA Medium |
JP6304-250G |
50 |
Minimal Media
基本培养基 |
Minimal SD Agar Base |
JP6311-467G |
46.7 |
Minimal SD Base |
JP6312-267G |
26.7 |
Minimal SD Agar Base Gal/Raf |
JP6313-270G |
54 |
Minimal SD Base Gal/Raf |
JP6314-185G |
37 |
Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His |
JP6316-10G |
1.29 |
DO Supplement -Leu |
JP6317-10G |
1.29 |
DO Supplement -Trp |
JP6318-10G |
1.29 |
DO Supplement -Ura |
JP6319-10G |
1.29 |
Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu |
JP6320-10G |
1.19 |
DO Supplement -His/-Ura |
JP6321-10G |
1.27 |
DO Supplement -Leu/-Trp |
JP6322-10G |
1.19 |
DO Supplement -Met/-Trp |
JP6323-10G |
1.27 |
DO Supplement -Trp/-Ura |
JP6324-10G |
1.27 |
DO Supplement -Trp/-His |
JP6325-10G |
1.27 |
DO Supplement -Ura/-Leu |
JP6326-10G |
1.27 |
Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp |
JP6331-10G |
1.17 |
DO Supplement -His/-Leu/-Ura |
JP6332-10G |
1.17 |
DO Supplement -His/-Trp/-Ura |
JP6333-10G |
1.25 |
DO Supplement -Leu/-Met/-Trp |
JP6334-10G |
1.17 |
DO Supplement -Leu/-Trp/-Ura |
JP6335-10G |
1.17 |
DO Supplement -Trp/-His/-Ade |
JP6336-10G |
1.17 |
Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp |
JP6341-10G |
1.15 |
DO Supplement -His/-Leu/-Met/-Trp |
JP6342-10G |
1.15 |
DO Supplement -His/-Leu/-Trp/-Ura |
JP6343-10G |
1.15 |
SD + DO Broth酵母缺陷型完全培养基(肉汤) |
SD/-His Broth |
JP6361-5L |
28 |
SD/-Leu Broth |
JP6363-5L |
28 |
SD/-Trp Broth |
JP6365-5L |
28 |
SD/-Ura Broth |
JP6367-5L |
28 |
SD/-His/-Leu Broth |
JP6381-5L |
28 |
SD/-His/-Ura Broth |
JP6383-5L |
28 |
SD/-Leu/-Trp Broth |
JP6385-5L |
28 |
SD/-Met/-Trp Broth |
JP6387-5L |
28 |
SD/-Trp/-Ura Broth |
JP6389-5L |
28 |
SD/-Trp/-His Broth |
JP6391-5L |
28 |
SD/-Trp/-Ade Broth |
JP6393-5L |
28 |
SD/-Ura/-Leu Broth |
JP6395-5L |
28 |
上接附表1酵母培养基配制浓度表
培养基类型 |
名称 |
货号 |
终浓度(g/L) |
SD + DO Broth酵母缺陷型完全培养基(肉汤) |
SD/-His/-Leu/-Trp Broth |
JP6411-5L |
28 |
SD/-His/-Leu/-Ura Broth |
JP6413-5L |
28 |
SD/-His/-Trp/-Ura Broth |
JP6415-5L |
28 |
SD/-Leu/-Met/-Trp Broth |
JP6417-5L |
28 |
SD/-Leu/-Trp/-Ura Broth |
JP6419-5L |
28 |
SD/-Trp/-His/-Ade Broth |
JP6421-5L |
28 |
SD/-Ade/-His/-Leu/-Trp Broth |
JP6431-5L |
28 |
SD/-His/-Leu/-Met/-Trp Broth |
JP6433-5L |
28 |
SD/-His/-Leu/-Trp/-Ura Broth |
JP6435-5L |
28 |
SD + DO with Agar酵母缺陷型完全培养基(琼脂) |
SD/-His with Agar |
JP6362-5L |
—— |
SD/-Leu with Agar |
JP6364-5L |
—— |
SD/-Trp with Agar |
JP6366-5L |
—— |
SD/-Ura with Agar |
JP6368-5L |
—— |
SD/-His/-Leu with Agar |
JP6382-5L |
—— |
SD/-His/-Ura with Agar |
JP6384-5L |
—— |
SD/-Leu/-Trp with Agar |
JP6386-5L |
—— |
SD/-Met/-Trp with Agar |
JP6388-5L |
—— |
SD/-Trp/-Ura with Agar |
JP6390-5L |
—— |
SD/-Trp/-His with Agar |
JP6392-5L |
—— |
SD/-Trp/-Ade with Agar |
JP6394-5L |
—— |
SD/-Ura/-Leu with Agar |
JP6396-5L |
—— |
SD/-His/-Leu/-Trpwith Agar |
JP6412-5L |
—— |
SD/-His/-Leu/-Urawith Agar |
JP6414-5L |
—— |
SD/-His/-Trp/-Urawith Agar |
JP6416-5L |
—— |
SD/-Leu/-Met/-Trpwith Agar |
JP6418-5L |
—— |
SD/-Leu/-Trp/-Urawith Agar |
JP6420-5L |
—— |
SD/-Trp/-His/-Adewith Agar |
JP6422-5L |
—— |
SD/-Ade/-His/-Leu/-Trpwith Agar |
JP6432-5L |
—— |
SD/-His/-Leu/-Met/-Trpwith Agar |
JP6434-5L |
—— |
SD/-His/-Leu/-Trp/-Urawith Agar |
JP6436-5L |
—— |
规格信息
品牌: |
Jinpan
|
CAS: |
N/A
|
规格: |
10×0.5 L
|
货期: |
咨询客服
|