N-Acetyl-L-Glutamic Acid N-乙酰-L-谷氨酸
货号: JP0451-25G 品牌: Jinpan 标签: 生化试剂
规格信息
品牌: |
Jinpan |
---|---|
CAS: |
1188-37-0 |
规格: |
25g |
货期: |
咨询客服 |
N-Acetyl-L-Glutamic Acid N-乙酰-L-谷氨酸
货号: JP0451-25G 品牌: Jinpan 标签: 生化试剂
品牌: |
Jinpan |
---|---|
CAS: |
1188-37-0 |
规格: |
25g |
货期: |
咨询客服 |
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针
货号: JP5203-25MG 品牌: Jinpan 标签: 细胞生物学研究
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针
(Amplex® Red H2O2 probe 过氧化氢探针)
搜索关键词:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP);Amplex®Red; Resorufin 试卤灵;过氧化氢探针;Ampliflu™ Red;CAS NO:119171-73-2;
基本介绍:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP),中文名10-乙酰基-3,7-二羟基吩嗪,以商标名Amplex®Red在市场上销售。ADHP是一种高度灵敏和稳定的过氧化物酶底物,选择性检测过氧化氢(H2O2)。存在辣根过氧化物酶(HRP)的体系中,这一基本无色和非荧光的ADHP与H2O2以1:1的化学定量比反应,生成强红色荧光的试卤灵(Resorufin)(Ex/Em= 571/585nm),可通过荧光光度计和酶标仪分析结果。许多酶反应能产生H2O2,因此,ADHP能够用在偶联酶促反应中检测许多不同酶的生物活性。
工作原理:
Fig 1. 使用ADHP进行偶联酶促反应的工作原理。葡萄糖氧化酶催化葡萄糖氧化生成过氧化氢(H2O2),通过辣根过氧化物酶(HRP)催化ADHP反应生成强荧光试卤灵(Resorufin)。
产品应用:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP)是目前位置最灵敏和稳定的荧光探针用来检测HRP和H2O2。
A) ADHP普遍用来检测许多免疫实验中的HRP;
B) ADHP用来检测微量的H2O2;
Zhou, M.等研究表明ADHP(Amplex®Red)检测过氧化物酶活性,最少能检测1×10−5U/mL HRP;或H2O2,最少能检测50nM H2O2。【文献来源:Zhou M et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem. 1997 Nov 15;253(2):162-8.】
基本特性:
1) CAS NO.:119171-73-2
2) 化学名:1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone; 10H-Phenoxazine-3,7-diol, 10-acetyl-;
3) 同义名:N-Acetyl-3,7-dihydroxyphenoxazine N-乙酰基-3,7-二羟基吩嗪;ADHP;Amplex®Red Reagent;A 6550;Ampliflu™ Red 荧光红染料;
4) 分子式:C14H11NO4
5) 分子量:257.25
6) 纯度:≥98%
7) 摩尔消光系数(ε):54,000 (Resorufin)
8) Ex/Em:571/585nm (pH 9.0, Resorufin)
9) 溶解性:溶于DJPO、DMF
10) 化学结构式:
ADHP的应用实例:
文献来源:Summers FA et al. Photooxidation of Amplex Red to resorufin: implications of exposing the Amplex Red assay to light. Methods Enzymol. 2013;526:1-17.
实验1)Amplex Red Assay
Reactions containing 50 µM Amplex Red reagent, 0.1 U/mL HRP, H2O2 and 0.5 U/mL catalase in 50 mM sodium phosphate buffer, pH 7.4, as indicated in the figure legends, were prepared in a darkroom under a red light. White ELISA plates containing the samples (100 µL/well) in triplicate were either kept in the dark for 30 and 60 min or under ambient laboratory light. The fluorescence (excitation 535 nm, emission 595 nm) was measured on a GENios platereader (Tecan, Durham, NC, USA).
实验2)Cell Culture Assay
HaCaT keratinocytes were incubated with 50 µM Amplex Red in PBS for 1 h at 37°C in the dark, then cells were irradiated using UVA (315–400 nm) from four fluorescent PUVA lamps (Houvalite F20T12BL-HO; National Biological Co., Twinsburg, OH) for 15 minutes (3.75 J/cm2) and 30 minutes (7.5 J/cm2). Control cells were treated with the same conditions. After exposure, the fluorescence (excitation 535 nm, emission 595 nm) was recorded using a plate reader.
产品订购:进口原料,现货供应,欢迎来电咨询021-50837765,或QQ:2971634497,1563822900。
货号 | 产品名称 | 规格 | 价格(元) |
JP5203-1MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 1mg | 339 |
JP5203-5MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 5mg | 1429 |
JP5203-25MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 25mg | 4489 |
注意事项
1) ADHP的使用比较方便,可配制10-20mM DJPO储存液,之后用反应缓冲液比如PBS,Tris buffer稀释到50-200µM工作浓度用于检测。最佳的工作浓度需根据自身实验需求进行优化。
2) Amplex®Red是Life Technologies公司的注册商标名。
3) 为了您的安全和健康,请穿实验服并戴一次性手套操作。
品牌: |
Jinpan |
---|---|
CAS: |
119171-73-2 |
规格: |
25mg |
货期: |
咨询客服 |
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针
货号: JP5203-5MG 品牌: Jinpan 标签: 细胞生物学研究
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针
(Amplex® Red H2O2 probe 过氧化氢探针)
搜索关键词:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP);Amplex®Red; Resorufin 试卤灵;过氧化氢探针;Ampliflu™ Red;CAS NO:119171-73-2;
基本介绍:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP),中文名10-乙酰基-3,7-二羟基吩嗪,以商标名Amplex®Red在市场上销售。ADHP是一种高度灵敏和稳定的过氧化物酶底物,选择性检测过氧化氢(H2O2)。存在辣根过氧化物酶(HRP)的体系中,这一基本无色和非荧光的ADHP与H2O2以1:1的化学定量比反应,生成强红色荧光的试卤灵(Resorufin)(Ex/Em= 571/585nm),可通过荧光光度计和酶标仪分析结果。许多酶反应能产生H2O2,因此,ADHP能够用在偶联酶促反应中检测许多不同酶的生物活性。
工作原理:
Fig 1. 使用ADHP进行偶联酶促反应的工作原理。葡萄糖氧化酶催化葡萄糖氧化生成过氧化氢(H2O2),通过辣根过氧化物酶(HRP)催化ADHP反应生成强荧光试卤灵(Resorufin)。
产品应用:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP)是目前位置最灵敏和稳定的荧光探针用来检测HRP和H2O2。
A) ADHP普遍用来检测许多免疫实验中的HRP;
B) ADHP用来检测微量的H2O2;
Zhou, M.等研究表明ADHP(Amplex®Red)检测过氧化物酶活性,最少能检测1×10−5U/mL HRP;或H2O2,最少能检测50nM H2O2。【文献来源:Zhou M et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem. 1997 Nov 15;253(2):162-8.】
基本特性:
1) CAS NO.:119171-73-2
2) 化学名:1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone; 10H-Phenoxazine-3,7-diol, 10-acetyl-;
3) 同义名:N-Acetyl-3,7-dihydroxyphenoxazine N-乙酰基-3,7-二羟基吩嗪;ADHP;Amplex®Red Reagent;A 6550;Ampliflu™ Red 荧光红染料;
4) 分子式:C14H11NO4
5) 分子量:257.25
6) 纯度:≥98%
7) 摩尔消光系数(ε):54,000 (Resorufin)
8) Ex/Em:571/585nm (pH 9.0, Resorufin)
9) 溶解性:溶于DJPO、DMF
10) 化学结构式:
ADHP的应用实例:
文献来源:Summers FA et al. Photooxidation of Amplex Red to resorufin: implications of exposing the Amplex Red assay to light. Methods Enzymol. 2013;526:1-17.
实验1)Amplex Red Assay
Reactions containing 50 µM Amplex Red reagent, 0.1 U/mL HRP, H2O2 and 0.5 U/mL catalase in 50 mM sodium phosphate buffer, pH 7.4, as indicated in the figure legends, were prepared in a darkroom under a red light. White ELISA plates containing the samples (100 µL/well) in triplicate were either kept in the dark for 30 and 60 min or under ambient laboratory light. The fluorescence (excitation 535 nm, emission 595 nm) was measured on a GENios platereader (Tecan, Durham, NC, USA).
实验2)Cell Culture Assay
HaCaT keratinocytes were incubated with 50 µM Amplex Red in PBS for 1 h at 37°C in the dark, then cells were irradiated using UVA (315–400 nm) from four fluorescent PUVA lamps (Houvalite F20T12BL-HO; National Biological Co., Twinsburg, OH) for 15 minutes (3.75 J/cm2) and 30 minutes (7.5 J/cm2). Control cells were treated with the same conditions. After exposure, the fluorescence (excitation 535 nm, emission 595 nm) was recorded using a plate reader.
产品订购:进口原料,现货供应,欢迎来电咨询021-50837765,或QQ:2971634497,1563822900。
货号 | 产品名称 | 规格 | 价格(元) |
JP5203-1MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 1mg | 339 |
JP5203-5MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 5mg | 1429 |
JP5203-25MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 25mg | 4489 |
注意事项
1) ADHP的使用比较方便,可配制10-20mM DJPO储存液,之后用反应缓冲液比如PBS,Tris buffer稀释到50-200µM工作浓度用于检测。最佳的工作浓度需根据自身实验需求进行优化。
2) Amplex®Red是Life Technologies公司的注册商标名。
3) 为了您的安全和健康,请穿实验服并戴一次性手套操作。
品牌: |
Jinpan |
---|---|
CAS: |
119171-73-2 |
规格: |
5mg |
货期: |
咨询客服 |
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针
货号: JP5203-1MG 品牌: Jinpan 标签: 细胞生物学研究
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针
(Amplex® Red H2O2 probe 过氧化氢探针)
搜索关键词:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP);Amplex®Red; Resorufin 试卤灵;过氧化氢探针;Ampliflu™ Red;CAS NO:119171-73-2;
基本介绍:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP),中文名10-乙酰基-3,7-二羟基吩嗪,以商标名Amplex®Red在市场上销售。ADHP是一种高度灵敏和稳定的过氧化物酶底物,选择性检测过氧化氢(H2O2)。存在辣根过氧化物酶(HRP)的体系中,这一基本无色和非荧光的ADHP与H2O2以1:1的化学定量比反应,生成强红色荧光的试卤灵(Resorufin)(Ex/Em= 571/585nm),可通过荧光光度计和酶标仪分析结果。许多酶反应能产生H2O2,因此,ADHP能够用在偶联酶促反应中检测许多不同酶的生物活性。
工作原理:
Fig 1. 使用ADHP进行偶联酶促反应的工作原理。葡萄糖氧化酶催化葡萄糖氧化生成过氧化氢(H2O2),通过辣根过氧化物酶(HRP)催化ADHP反应生成强荧光试卤灵(Resorufin)。
产品应用:
10-Acetyl-3,7-dihydroxyphenoxazine (ADHP)是目前位置最灵敏和稳定的荧光探针用来检测HRP和H2O2。
A) ADHP普遍用来检测许多免疫实验中的HRP;
B) ADHP用来检测微量的H2O2;
Zhou, M.等研究表明ADHP(Amplex®Red)检测过氧化物酶活性,最少能检测1×10−5U/mL HRP;或H2O2,最少能检测50nM H2O2。【文献来源:Zhou M et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem. 1997 Nov 15;253(2):162-8.】
基本特性:
1) CAS NO.:119171-73-2
2) 化学名:1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone; 10H-Phenoxazine-3,7-diol, 10-acetyl-;
3) 同义名:N-Acetyl-3,7-dihydroxyphenoxazine N-乙酰基-3,7-二羟基吩嗪;ADHP;Amplex®Red Reagent;A 6550;Ampliflu™ Red 荧光红染料;
4) 分子式:C14H11NO4
5) 分子量:257.25
6) 纯度:≥98%
7) 摩尔消光系数(ε):54,000 (Resorufin)
8) Ex/Em:571/585nm (pH 9.0, Resorufin)
9) 溶解性:溶于DJPO、DMF
10) 化学结构式:
ADHP的应用实例:
文献来源:Summers FA et al. Photooxidation of Amplex Red to resorufin: implications of exposing the Amplex Red assay to light. Methods Enzymol. 2013;526:1-17.
实验1)Amplex Red Assay
Reactions containing 50 µM Amplex Red reagent, 0.1 U/mL HRP, H2O2 and 0.5 U/mL catalase in 50 mM sodium phosphate buffer, pH 7.4, as indicated in the figure legends, were prepared in a darkroom under a red light. White ELISA plates containing the samples (100 µL/well) in triplicate were either kept in the dark for 30 and 60 min or under ambient laboratory light. The fluorescence (excitation 535 nm, emission 595 nm) was measured on a GENios platereader (Tecan, Durham, NC, USA).
实验2)Cell Culture Assay
HaCaT keratinocytes were incubated with 50 µM Amplex Red in PBS for 1 h at 37°C in the dark, then cells were irradiated using UVA (315–400 nm) from four fluorescent PUVA lamps (Houvalite F20T12BL-HO; National Biological Co., Twinsburg, OH) for 15 minutes (3.75 J/cm2) and 30 minutes (7.5 J/cm2). Control cells were treated with the same conditions. After exposure, the fluorescence (excitation 535 nm, emission 595 nm) was recorded using a plate reader.
产品订购:进口原料,现货供应,欢迎来电咨询021-50837765,或QQ:2971634497,1563822900。
货号 | 产品名称 | 规格 | 价格(元) |
JP5203-1MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 1mg | 339 |
JP5203-5MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 5mg | 1429 |
JP5203-25MG | 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) 过氧化氢探针 | 25mg | 4489 |
注意事项
1) ADHP的使用比较方便,可配制10-20mM DJPO储存液,之后用反应缓冲液比如PBS,Tris buffer稀释到50-200µM工作浓度用于检测。最佳的工作浓度需根据自身实验需求进行优化。
2) Amplex®Red是Life Technologies公司的注册商标名。
3) 为了您的安全和健康,请穿实验服并戴一次性手套操作。
品牌: |
Jinpan |
---|---|
CAS: |
119171-73-2 |
规格: |
1mg |
货期: |
咨询客服 |
简要描述:4-Nitrophenyl-N-acetyl-ß-D-galactosaminide – 100 mg,产品编码:AS-85618。品牌:ANASPEC显色 N-乙酰氨基半乳糖苷酶底物.
详细介绍
产品咨询
品牌 | 其他品牌 | 供货周期 | 一个月 |
---|---|---|---|
应用领域 | 医疗卫生,环保,化工,生物产业,综合 |
4-Nitrophenyl-N-acetyl-ß-D-galactosaminide – 100 mg,产品编码: AS-85618。品牌:ANASPEC
4-Nitrophenyl-N-acetyl-ß-D-galactosaminide – 100 mg,描述:
化学
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上海金畔生物科技有限公司
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