SiliaBond^® C1 (or C1) has the lowest degree of hydrophobicity of all reversed stationary phases, useful in the separation of large biomolecules that have extensive hydrophobic regions.

PRODUCT SPECIFICATIONS

MSDS-R33030B

This non-endcapped version of the SiliaBond^® C18 15.5% BDS, presents a perfect monolayer of dimethyloctadecylsilane and silanol functions.  The hydrophilic/hydrophobic balance is permitting to use a mobile phase with a higher concentration of water and ion pairing conditions for separation of biomolecules.

APPLICATION NOTES
Bulkor/Batch mode : Click here
SPE : Click here

POSTER
Click here to see all the documentation about SiliaBond^®

RELATED PUBLICATION

1. Neuroscience, 156 (2008) 466

SiliCycle is proud to offer this new base deactivated C18 , the SiliaBond^® C18 16% BDS. Last year SiliCycle developed a new and innovative C18 chromatographic phase chracterized by a homogeneous coverage of the alkyl chains on the surface. Consequently, the endcapping step is more controlled, which leads to much improved separations and also to inhibate the non-specific interactions with silanol. This chromatographic phase is available on irregular (R332-) and spherical (S032-) high quality supports.

In reversed-phase chromatography, the packing is always non-polar (hydrophobic) while the mobile phase is polar to non-polar. An important parameter affecting the chromatographic efficiency is the hydrophobicity of the sorbent. As ageneral rule, stationary phase hydrophobicity increases with the alkyl chain length.

Compare to competitive products, this endcapped C18 16% BDS exhibits high hydrophobicity and base deactivated prperties. We have compared this new chromatographic phase to comparable 20 % C18 phases on the market. The comparison was done on a mixture of compounds to evaluate the dead volume (uracil), the hydrophobicity (toluene and biphenyl) and silanol activity (amitriptyline). The test was done in isocratic conditions, with a mobile phase composed of 80/20 methanol/buffer (20 nM potassium phosphate pH = 7). The results are presented below:

APPLICATION NOTES
Bulkor/Batch mode : Click here
SPE : Click here

POSTER
Click here to see all the documentation about SiliaBond^®

RELATED PUBLICATION

1. Neuroscience, 156 (2008) 466

SiliaBond^® C4 (or C4) provides less retention towards non-polar compounds than C18 and C8, and is useful in ion-pairing chromatography or to separate large biomolecules.  It works best for molecules with a large hydrophilic region or in case where the hydrophobic region is buried within the structure.

POSTER
Click here to see all the documentation about SiliaBond^®