订购满5000元，200元礼品等你拿

凑单关联产品TOP5

NO.1.    ROS Assay Kit -Highly Sensitive DCFH-DA-    ROS检测

NO.2.    Annexin V, FITC Apoptosis Detection Kit    细胞凋亡检测

NO.3.    Calcein-AM/PI Double Staining Kit    活死细胞双染检测

NO.4.    Cell Cycle Assay Kit    细胞周期检测

NO.5.    Caspase-3 Assay Kit-Colorimetric-    细胞凋亡检测

细胞毒性检测通常通过检测活细胞数量或者死细胞数量来确定。而为了更加全面的评价细胞毒性情况，多种评价方法相互验证的需求与日俱增。Viability/CytotoxicityMultiplex Assay Kit可以综合评价细胞毒性。试剂盒内含Cell Counting Kit-8 (CCK-8) 和Cytotoxicity LDH Assay Kit-WST^® (LDH Assay Kit)，分别用于测定活细胞和死细胞数量。

CCK-8通过检测活细胞中脱氢酶活性来确定活细胞数，而LDH Assay Kit通过检测受损细胞膜释放LDH的量来确定死细胞数。LDH Assay Kit可以直接添加到含有细胞的培养基中检测 (一步法)。也可以分离细胞后，加到细胞培养基上清液中检测 (低损伤法，分离的细胞可以做其它实验)。两种细胞毒性评价指标，CCK-8和LDH Assay Kit(低损伤法) 可以使用同一批细胞同时进行实验。

不同细胞毒性指标，双重印证。增加结果可信性。

LDH Assay Kit：细胞膜损伤，LDH外漏，通过检测LDH活性判断细胞毒性。

CCK-8：可以使用NADH作为指标来确认活细胞中的代谢活性。

本试剂盒包括用于测量活细胞的CCK-8和用于测量死细胞的LDH分析试剂盒，两者均可通过平板分析法在相同的吸收波长下进行评估。 此外，同一细胞样本可用于同时评估活细胞和死细胞，从而节省了时间和精力。

本产品作为CCK-8（500 tests）与Cytotoxicity LDH Assay Kit（500 tests）组合套装，低于单独购买的总价。适合新接触细胞毒性检测的用户，用于前期摸索实验条件。单独产品链接见下方。大包装购买，单价会降低很多。

Cell Counting Kit-8

Cytotoxicity LDH Assay Kit-WST

1. Integrated Omics Reveals Tollip as an Regulator and Therapeutic Target for Hepatic Ischemia-Reperfusion Injury in Mice,

Hepatology., 2019, doi: 10.1002/hep.30705

2. Amplified Cancer Immunotherapy of Surface Engineering Antigenic Microparticles Vaccine by Synergistically Modulating Tumor

Microenvironment, ACS Nano, 2019, 13, 11, 12553-12566

3. A novel IFNα-induced long noncoding RNA negatively regulates immunosuppression by interrupting H3K27 acetylation in head

and neck squamous cell carcinoma, Molecular Cancer, 2020,19, 4, doi: 10.1186/s12943-019-1123-y

4. Untangling the co-effects of oriented nanotopography and sustained anticoagulation in a biomimetic intima on neovessel

remodeling, Biomaterials, 2019, 119654

5. In Vitro and in Vivo Analysis of Mineralized Collagen-Based Sponges Prepared by a Plasma- and Precursor-Assisted

Biomimetic Process, ACS Applied Materials & Interfaces, 2017, 9, 27, 22185-22194

6. MoS2-LA-PEI nanocomposite carrier for real-time imaging of ATP metabolism in glioma stem cells co-cultured with

endothelial cells on a microfluidic system, Biosensors and Bioelectronics, 2018, 99, 142-149

7. Biocompatible carbon nanotube fiber for implantable supercapacitors, Carbon, 2017, 122, 162-167

8. Genomic sequencing and editing revealed the GRM8 signaling pathway as potential therapeutic targets of squamous cell

lung cancer, Cancer Letters, 2019, 442, 53-67

9. Knockdown of TGF-β1 expression in human umbilical cord mesenchymal stem cells reverts their exosome-mediated EMT

promoting effect on lung cancer cells, Cancer Letters, 2018, 428:34-44

10. Restriction of exogenous DNA expression by SAMHD1, Science Bulletin, 2020, https://doi.org/10.1016/j.scib.2019.12.028

11. PARP1 inhibition alleviates injury in ARH3-deficient mice and human cells, JCI Insight, 2019, 4(4), e124519

12. Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in

triple-negative breast cancer, Cell Death & Disease, 2018, 9(2), 137

13. Ischemic preconditioning attenuates ischemia/reperfusion-induced kidney injury by activating autophagy via the SGK1

signaling pathway, Cell Death & Disease, 2018, 9, 338

14. Pathological Endogenous a-Synuclein Accumulation in Oligodendrocyte Precursor Cells Potentially Induces Inclusions in

Multiple System Atrophy, Stem Cell Reports, 2018, 10(2), 356-365

15. PDH-mediated metabolic flow is critical for skeletal muscle stem cell differentiation and myotube formation during regeneration

in mice, FASEB Journal, 2019, 33(7), 8094-8109

16. Benzo[a]pyrene-decreased gap junctional intercellular communication via calcium/calmodulin signaling increases apoptosis in

TM4 cells, Journal of Applied Toxicology, 2018, 38(8), 1091-1103

订购满5000元，200元礼品等你拿

凑单关联产品TOP5

NO.1.    Cell Counting Kit-8    细胞增殖毒性检测

NO.2.    Cytotoxicity LDH Assay Kit-WST    乳酸脱氢酶(LDH)检测

NO.3.    Caspase-3 Assay Kit-Colorimetric-    细胞凋亡检测

NO.4.    Annexin V, FITC Apoptosis Detection Kit    细胞凋亡检测

NO.5.    ROS Assay Kit -Highly Sensitive DCFH-DA-    ROS检测

ATP是生物体内最直接的能量来源，在肌肉收缩、代谢反应、主动运输等方面被广泛使用，甚至被称作生物体内的能量货币。同仁化学研究所开发的Cell Counting Kit-Luminescence试剂盒是一种通过Luciferase来确定细胞中的腺苷三磷酸（ATP）的细胞增殖-毒性检测试剂盒。

本试剂盒只需将各试剂混合后加入孔板，10 分钟后即可检测。不需要去除培养基、清洗细胞等复杂的操作。此外，本试剂盒还有诸如发光的半衰期在3 小时以上、数据的重现性高 、兼容96孔板 、384孔板的多样品检测等诸多优点。

特点1.微量细胞的高灵敏度快速检测

在相同实验条件下（1000 cells/well HeLa细胞，经过连续2倍稀释，制成500 cells, 250 cells, 125 cells, 63 cells, 31 cells, 16 cells细胞悬液），CCK-L经10 min培养即可获得高灵敏度结果，0cell(RLU 59)和16cells(RLU 900)的发光值的差距约15倍！而CCK-8即使经过3 h的培养，虽然标准曲线的相关系数仍然很高（0.9982），但是实验孔与空白孔之间的差值相对较小。

特点2.发光信号稳定持久

图3.A549细胞3小时内发光信号检测实例

白色96孔板各种细胞的检测示例

各细胞进行2倍梯度稀释制成细胞悬液。白色96孔板中，每孔加入100 μl细胞悬液，过夜培养后加入相同体积的Working soluiton，震荡后静置10 min，检测发光值。

*HeLa：含10% FBS的MEM培养基；A549：含10% FBS的DMEM培养基；Jurkat：含10% FBS的RPMI培养基；HEK293：含10% FBS的DMEM培养基；HL 60: 含10% FBS的RPMI培养基。