Glutaminase(GLN) 谷氨酰胺酶| 龟甲万百欧凯米发株式会社

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Glutaminase(GLN) 谷氨酰胺酶

  • 临床检测用酶

The enzyme is useful for the determination of L-glutamine in clinical analysis.

由来 from microorganism
系统名称

L-Glutamine amidohydrolase

反应式

L-Glutamine + H2O →→→ L-Glutamineate + NH3

规格

Appearance light gray lyophilizate
Activity ≧4 U/mg
Contaminants Catalase ≦3.0 U/U%
Stabilizer sucrose
Storage condition below – 20℃

特性

Molecular weight ca. 58 kDa (gel filtration)
Structure heterodimer (45 kDa and 22 kDa) (SDS-PAGE)
Michaelis constant  5.5×10-4 M (L-glutamine)
pH Optimum ca. 7.0
pH Stability 5.5–9.0
Optimum temperature ca. 65℃
Thermal stability below 50℃
Stability (liquid form) stable at 37℃ for at least four days
Stability (powder form) stable at 30℃ for at least two weeks
Specificity L-glutamine (100), D-glutamine (70), L-asparagine (0), D-asparagine (0)

适用类型

The enzyme is useful for the determination of L-glutamine in clinical analysis.

参考文献

Roberts, E., “The Enzymes,” Vol. 4 (2nd ed.), Academic Press, New York and London, 1960, pp. 285–300.

Hartman, S. C., “The Enzymes,” Vol. 4 (3rd ed.), Academic Press, New York and London, 1971, pp. 79–100.

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Glutamine Synthetase (GST) 谷氨酰胺合成酶| 龟甲万百欧凯米发株式会社

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Glutamine Synthetase (GST) 谷氨酰胺合成酶

  • 临床检测用酶

The enzyme is useful for the determination of ammonia and ATP in clinical analysis.

由来 microorganism
系统名称

L-Glutamate : ammonia ligase (ADP-forming)

EC编号 6.3.1.2
反应式

ATP + L-Glutamate + NH3→→→ ADP + Orthophosphate + L-Glutamine

规格

Appearance white to light yellow lyophilizate
Activity ≧15.0 U/mg
Contaminants Catalase ≦0.5 U/U%
Stabilizer sucrose
Storage condition below-20℃

特性

Molecular weight ca. 900 kDa (gel filtration)
Structure 57 kDa (SDS-PAGE)
Isoelectric point 6.5
Michaelis constant 1.5×10-2M (L-glutamate)
1.3×10-4M (ammonia)
8.7×10-4M (ATP)
pH Optimum ca. 7.0
pH Stability 6.5–9.5
Optimum temperature 60℃
Thermal stability below 40℃
Inhibitors methionine sulfoximine, carbamyl phosphate
Activators Mg2+,Mn2+
Specificity L-glutamate (100), D-glutamate (0.8)
NH₃(100), NH₂OH (12)
ATP (100), GTP (2.5)

适用类型

The enzyme is useful for the determination of ammonia and ATP in clinical analysis.

参考文献

Ebner, E. et al., “Methods in Enzymology,” Vol. 17A, Academic Press, New York, 1970, pp. 910–922.

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Millipore密理博 Pellicon® XL 装置

Millipore密理博 Pellicon® XL 装置

简要描述:

Pellicon XL 是一个用于制药与生物技术开发应用的小容量切向流器 (TFF)。欢迎客户“Millipore密理博 Pellicon® XL 装置“详细说明书。

Pellicon XL 是一个用于制药与生物技术开发应用的小容量切向流器 (TFF)。

技术指标

Pellicon XL 装置
结构材质
Biomax 膜 改良聚醚砜
Ultracel 膜 再生纤维素
屏幕,外壳,连接器, Luer 盖 聚丙烯
连接 阴 Luer
过滤面积, cm2 50
尺寸
长度,cm (in) 18.8 (7.4)
宽度,cm (in) 3 (1.2)
滞留量(典型), mL 3.2
zui大进口压力,bar (psi) 5.5 (80) at 50°C
zui大膜透压,bar (psig) 2.8 (40)
Labscale TFF 系统
尺寸
长度,cm (in) 21.6 (8.5)
宽度,cm (in) 27.9 (11)
高度,cm (in) 45.7 (18)
重量,kg (lb) 5.9 (13)
zui大进口压力,bar (psi)  
进给流速,mL/min,4 bar (60psi) 10 至 100
zui小循环体积, mL  
操作温度范围,°C 4–45

技术参数

Pellicon XL 装置
结构材质
Biomax 膜 改良聚醚砜
Ultracel 膜 再生纤维素
屏幕,外壳,连接器, Luer 盖 聚丙烯
连接 阴 Luer
过滤面积, cm2 50
尺寸
长度,cm (in) 18.8 (7.4)
宽度,cm (in) 3 (1.2)
滞留量(典型), mL 3.2
zui大进口压力,bar (psi) 5.5 (80) at 50°C
zui大膜透压,bar (psig) 2.8 (40)
Labscale TFF 系统
尺寸
长度,cm (in) 21.6 (8.5)
宽度,cm (in) 27.9 (11)
高度,cm (in) 45.7 (18)
重量,kg (lb) 5.9 (13)
zui大进口压力,bar (psi)  
进给流速,mL/min,4 bar (60psi) 10 至 100
zui小循环体积, mL  
操作温度范围,°C 4–45

Lactate Dehydrogenase (LDH-P) 乳酸脱氢酶| 龟甲万百欧凯米发株式会社

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Lactate Dehydrogenase (LDH-P) 乳酸脱氢酶

  • 临床检测用酶

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate,lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

由来 porcine heart
系统名称

(S )-Lactate : NAD+ oxidoreductase

EC编号 1.1.1.27
反应式

Pyruvate + NADH + H+ L-Lactate + NAD+

规格

Appearance white lyophilizate
Activity ≧150 U/mg
Storage condition below -20℃

特性

Molecular weight ca. 115 kDa (gel filtration)
Michaelis constant 2.5×10-2M (fructosyl-glycine)
1.0×10-4M (pyruvate)
pH Optimum 7.4–8.0
pH Stability 7.0–7.5
Optimum temperature 50–55℃
Thermal stability below 45℃

适用类型

The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.

参考文献

  • Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
  • Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
  • Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
  • Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).
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