Cell Navigator Lysosome Staining Kit *Orange Fluorescence*
Cell Navigator溶酶体标记试剂盒 *橙色荧光*
货号: 22657 品牌: AAT Bioquest 标签: 有机试剂
规格信息
品牌: |
AAT Bioquest |
---|---|
CAS: | |
规格: |
1 kit |
货期: |
5-7天(参考) |
Cell Navigator Lysosome Staining Kit *Orange Fluorescence*
Cell Navigator溶酶体标记试剂盒 *橙色荧光*
货号: 22657 品牌: AAT Bioquest 标签: 有机试剂
品牌: |
AAT Bioquest |
---|---|
CAS: | |
规格: |
1 kit |
货期: |
5-7天(参考) |
Millipore密理博Millex-FA50针式过滤器
Millipore密理博Millex-FA50针式过滤器, 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 10/PK
SLFA05010
Millex 过滤器
装有疏水性 Fluoropore 膜的 Millex 过滤器非常适合于气体除菌、无菌容器换气及有机溶液除菌或澄清过滤。 特制的过滤器可以保护血液透析换能器不会受到血液和湿气的影响。
说明: Millex-FA,1.0 µm,疏水性 PTFE,50 mm,HB-HB
商标名: Millex
数量/包装: 100
应用: 澄清非水溶液、真空管保护、空气过滤及非无菌储液罐和生物反应器换气
进口接头: 阶形倒刺软管接口,带阴 Luer 内滑动接头
滤膜材质: Hydrophobic PTFE
色码: 进/出口均为自然色
滤膜商标名: Fluoropore
zui大进口压力,bar (psi): 4.1 (60)
出口接头: 阶形倒刺软管接口,带阴 Luer 内滑动接头
zui高操作温度,°C: 121
滤膜孔径,µm: 1.0
可润湿性: 疏水
灭菌: 可高压高温灭菌
直径,mm: 62
滤膜直径,mm: 50
高度,mm: 71
产品名称: Millex-FA50 过滤器
过滤面积,cm2: 19.6
无菌: 非无菌
技术指标
50mm Millex
外壳材料
过滤面积,cm2 19.6
zui大进口压力,bar (psi) 4.1 (60)
zui高操作温度,°C 121比较
Millipore密理博Millex-FA50针式过滤器 SLFA05010
产品详情:
目录编号 产品名称 滤膜直径,mm 滤膜孔径,µm 进口接头 出口接头 灭菌 数量/包装
SLFG025LS Millex-FG 过滤器 25 0.2 阴 Luer-Lok 接头 阳 luer 滑动接头 环氧乙烷 50
SLFGL25BS Millex-FG 过滤器 25 0.2 阴 Luer-Lok 接头 阳 luer 滑动接头 环氧乙烷 50
SLFG02550 Millex-FG 过滤器 25 0.2 阴 Luer-Lok 接头 阳 luer 滑动接头 可高压高温灭菌 50
SLFG05010 Millex-FG50 过滤器 62 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 Stepped Hose Barb with female Luer slip interior 可高压高温灭菌 10
SLFG05000 Millex-FG50 过滤器 50 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 100
SLFG55010 Millex-FG50 过滤器 50 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 1/8″ NPTM 可高压高温灭菌 10
SLFG65010 Millex-FG50 过滤器 50 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 1/8″ NPTM 可高压高温灭菌 10
SLFG65000 Millex-FG50 过滤器 50 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 1/8″ NPTM 可高压高温灭菌 100
SLFG75010 Millex-FG50 过滤器 50 0.2 1/8″ NPTM 1/8″ NPTM 可高压高温灭菌 10
SLFG75000 Millex-FG50 过滤器 50 0.2 1/8″ NPTM 1/8″ NPTM 可高压高温灭菌 100
SLFG85010 Millex-FG50 过滤器 50 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 10
SLFG85000 Millex-FG50 过滤器 50 0.2 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 100
SLFH05010 Millex-FH50 过滤器 50 0.45 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 10
SLFH05000 Millex-FH50 过滤器 50 0.45 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 100
SLFA05000 Millex-FA50 过滤器 50 1.0 阶形倒刺软管接口,带阴 Luer 内滑动接头 阶形倒刺软管接口,带阴 Luer 内滑动接头 可高压高温灭菌 100
Trimethoprim 甲氧苄啶
货号: JP0046-5G 品牌: Jinpan 标签: 生化试剂
Trimethoprim 甲氧苄啶
产品关键词
Trimethoprim 甲氧苄啶;Sulfamethoxazole 磺胺甲恶唑;二氢叶酸还原酶(DHFR);Sulfadimethoxine 磺胺二甲氧嘧啶;Sulfonamide antibiotic 磺胺类抗生素;CAS:738-70-5;
产品信息
产品名称 |
产品编号 | 规格 | 价格(元) |
Trimethoprim 甲氧苄啶 |
JP0046-5G | 5g | 180 |
Trimethoprim 甲氧苄啶 | JP0046-25G | 25g |
292 |
Trimethoprim 甲氧苄啶 | JP0046-100G | 100g |
592 |
产品描述
甲氧苄啶(Trimethoprim)是一种合成抑菌剂,选择性抑制原核微生物的二氢叶酸还原酶(DHFR)活性。甲氧苄啶通常与磺胺甲恶唑(JP00010-5G)联合使用,抑制金黄色葡萄球菌的生长,作用机制在于通过阻碍核苷酸合成,干扰细菌内叶酸的细胞代谢。甲氧苄啶与DHFR结合,抑制二氢叶酸(DHF)和四氢叶酸(THF)还原,后者是胸腺嘧啶合成途径的基本前体,从而抑制核酸合成。
产品特性
CAS NO:738-70-5 | 化学名:5-[(3,4,5-trimethoxyphenyl)methyl]-2,4-pyrimidinediamine |
同义名:2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine, NIH 204, NSC 106568;
2,4-二氨基-5-(3,4,5-三甲氧基苄基)嘧啶,甲氧苄氨嘧啶,三甲氧苄氨嘧啶,三甲氧苄二氨嘧啶; |
|
分子式:C14H18N4O3 | 分子量:290.32 g/mol |
纯度:≥98% | 外观:白色至类白色粉末 |
抗菌谱:革兰氏阳性菌、革兰氏阴性菌、分枝杆菌 | 溶解性:溶于DJPO(50mg/ml),微溶于乙醇或丙酮,
几乎不溶于水(0.4mg/ml) |
化学结构式:
|
保存:2-8℃干燥保存,至少2年有效。
运输:室温运输。
注意事项
相关产品
货号 | 名称 | 规格 |
JP0046-5G | Trimethoprim 甲氧苄啶 | 5g |
JP0047-250MG | Trimethoprim Lactate Salt 甲氧苄啶乳酸盐 | 250mg |
JP00010-5G | Sulfamethoxazole 磺胺甲恶唑 | 5g |
品牌: |
Jinpan |
---|---|
CAS: |
738-70-5 |
规格: |
5g |
货期: |
咨询客服 |
FM 4-64 (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)
货号: JP4016-1MG 品牌: Jinpan 标签: 细胞生物学研究
产品关键词:
FM 4-64;FM 1-43;SynaptoRed C2;Lipophilic probe;Yeast vacuolar membranes 酵母液泡膜;Synaptic vesicle 突触小泡;
订购信息:
产品名称 |
产品编号 | 规格 | 价格(元) |
FM 4-64 (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) |
JP4016-100UG |
100μg | 1372 |
JP4016-200UG | 2×100μg |
2692 |
|
JP4016-1MG | 1mg |
3382 |
产品描述
FM 4-64,英文全名:N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide,是一种亲脂的苯乙烯染料,用作一种活细胞探针示踪酵母整体膜内在化和运输到液泡。FM 4-64是一种灵敏的液泡动力学分析探针,检测一系列相关事件,包括有丝分裂中的分离结构形成、液泡分裂和融合事件,以及液泡蛋白质分拣蛋白突变体在不同阶段的液泡形态。
FM 4-64具水溶性、对细胞无毒性,在水溶液中基本无荧光,一旦插入脂膜表面发出强的红色荧光(Ex/Em= 558/734nm)。FM 4-64与呈绿色荧光的FM 1-43(JP4014)在合适的滤片能够区分开,允许双色实时观察膜的再循环。
产品特性
1)同义名:N-(3-Triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide
2)分子式:C30H45Br2N3
3) 分子量:607.51
4) 纯度:≥90%(HPLC)
5) 外观:紫色固体
6) 溶解性:溶于水、DJPO
7) Ex/Em:558/734nm(与膜结合)
8) 溶解性:溶于水
9) 化学结构式:
保存与运输方法
保存:-20°C避光干燥保存,至少1年有效。
运输:冰袋运输。
注意事项
应用示例(来自文献,仅作参考)
文献一、Luo Wj et al. Novel Genes Involved in Endosomal Traffic in Yeast Revealed by Suppression of a Targeting-defective Plasma Membrane ATPase Mutant. J Cell Biol. 1997 Aug 25;138(4):731-46. PMID: 9265642
使用方法(FM 4-64 Endocytosis):For FM 4-64 internalization studies, cells were grown to mid-log phase in YPD. After resuspension in fresh YPD at 20 OD600/ml, 20 μM FM 4-64 was added for 5 min at 30°C. Cells were washed, and incubation continued at 30°C for 1 h. FM 4-64 fluorescence was observed with rhodamine fluorescence filter sets. 【使用FM 4-64的原理在于:FM 4-64是批量质膜内在化的一种标记探针,从细胞表面到液泡膜的内吞示踪的转运以一种时间、温度和能量依赖性的方式进行。】
Fig 1. Visualization of endocytosis in sop mutants by FM 4-64 staining. Exponentially growing wild-type cells and sop mutants were stained with FM 4-64 for 5 min at 30°C, washed, and incubated in fresh YPD for 1 h before visualization and photography. Vacuolar membrane staining is seen in wild-type cells (L3852). In vps36 (WLX12-7C), a class E vps mutant, FM 4-64 dye is accumulated in a prevacuolar compartment. A similar accumulation of dye as a spot near the vacuole is seen in vps38 (WLX14-10A) and vps13 (WLX15-4C). Bright punctate staining in the cytoplasm remains in vps8 (WLX16-1A) after 1 h of chase.
文献二、Masamitsu Shimazu et al. A Family of Basic Amino Acid Transporters of the Vacuolar Membrane from Saccharomyces cerevisiae.
使用方法(FM 4-64 stain for vacuolar membrane):Subcellular localization of Vba1p-GFP fusion protein in living S. cerevisiae cells was assessed using fluorescence microscopy. To stain vacuolar membranes, FM 4–64 was added to growing cultures to a final concentration of 5 μM. The cells were further cultured for 20 min and harvested. After washing, the cells were resuspended in fresh YPD media for 30 min to allow the dye to stain the vacuole via endocytosis.
FIG 2. Fluorescence microscopy of the transformant Δvba1/pVBA1GFP. A, GFP fluorescence; B, FM 4–64 fluorescence; C, Nomarski; D, merged image.
文献三、Sun Y et al. Scribble interacts with beta-catenin to localize synaptic vesicles to synapses. Mol Biol Cell. 2009 Jul;20(14):3390-400.
使用方法(FM 4-64 stain for presynaptic terminals):Briefly, 15 μM FM 4-64 was loaded for 30 s into presynatpic terminals using a hyperkalemic solution of 90 mM KCl in modified HBSS, where equimolar NaCl was omitted for final osmolality of 310 mOsm. Neurons were rinsed three times and maintained in HBSS without Ca2+ for imaging. ADVESAP-7 (1 mM) was added to quench the nonspecific signal. Three images were captured every 30 s to confirm that the positive FM 4-64 sites were stationary presynaptic terminals. Unloading was done using the hyperkalemic solution described above, and neurons were rinsed three times with NeuroBasal media for continued imaging.
Fig 3. Deficits in SV recycling after scribble knockdown. (A–F) Confocal images of 10 DIV neurons transfected with Syn-GFP and the indicated RNAi using Lipofectamine 2000 (<1% transfection efficiency). Neurons were loaded with FM 4-64, and three images were captured every 30 s to confirm that the positive FM 4-64 sites were stationary presynaptic terminals. Arrows indicate FM 4-64–positive sites on transfected axons. FM dyes were then unloaded to demonstrate specificity (A′–F′). (F′) The FM 4-64–positive site (arrowhead) not observed in dye-“load” image (E), but observed following dye-“unload” (E′) most likely represents a mobile FM 4-64–positive puncta on an untransfected neuron. The FM 4-64 cluster in the transfected neurons (arrow) is not observed after de-staining. The density (G) and size (H) of FM 4-64-positive puncta ± SE were reduced in cells expressing RNAi-3 compared with control. N = 17 cells and >85 FM-4-64 puncta from more than three separate cultures. *p < 0.05 using Student’s t test. Scale bar, 5 μm.
使用方法
于实验前,将冻干粉置于室温回温至少20min,加入无菌水或DJPO配制成10mM或其他浓度储存液,比如,对于1mg FM 4-64(Mw: 607.51)加入164μl DJPO,充分溶解后即得到10mM储存液,根据单次用量分装,≤-20℃冻存,避免反复冻融。
【注意】:
a)以下以爬片生长的贴壁活细胞的质膜染色为例,仅作参考。最佳的染色条件根据使用细胞特征进行调整。
b)由于FM 4-64快速被内吞,很有必要参考下方的温度和时间指导来减慢内吞,提高质膜的选择性标记和成像。内吞很可能在染色的10min内发生。
c)以下步骤推荐使用不含钙镁的HBSS(JP3505-500ML)。钙镁的存在明显会加速染料内吞,导致质膜选择性染色很弱。
2.1用提前冰浴的HBSS缓冲液来稀释储存液到所需的工作浓度比如8μM。
2.2 将盖玻片从培养基内取出,快速的浸入含足量FM 4-64的染色工作液,冰上孵育1min。质膜能被快速染色。
2.3 将盖玻片从染色工作液中取出,置于载玻片上封片,周围用石蜡密封,置于冰上,立即成像。
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——Written/Edited by V. Shallan【版权归集奇生物/JP金畔所有】
品牌: |
Jinpan |
---|---|
CAS: |
N/A |
规格: |
1mg |
货期: |
咨询客服 |
0.5-10ul盒装灭菌透明滤芯f吸头,96支/盒,10盒/大盒,5大盒/箱 货号: TF-400-R-S 品牌: Axygen 标签: 实验耗材
品牌: |
Axygen |
---|---|
CAS: |
N/A |
规格: |
10 盒 / 大盒, 5 大盒 / 箱 |
货期: |
现货1~3天 期货30天 |