IL-36α, Human, Recombinant


IL-36α, Human, Recombinant

货号:PCK194

价格:¥1780¥4980¥17480¥25680

规格:

10µg

  • 10µg
  • 50µg
  • 500µg
  • 1mg

  • 产品概述
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产品概述

产品信息

别名 Interleukin-36 Alpha; FIL1 Epsilon; Interleukin-1 Epsilon; IL-1 Epsilon; Interleukin-1 Family Member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6
物种 Human
表达宿主 E.coli
序列信息 asd
检索号 Q9UHA7
分子量 17.1 kDa
表观分子量 17 kDa
生物活性 Measured in a cell proliferation assay using A-431 Human epithelial carcinoma cells. The ED50 for this effect is 12.0 ng/ml.

产品特性

纯度 >95% as determined by reducing SDS-PAGE.
内毒素 < 1.0 EU per µg as determined by LAL test.
保存 Lyophilized Protein should be stored at < -20℃, though stable at room temperature for 3 weeks. Reconstituted Protein solution can be stored at 4-7℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输 The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
制剂 Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1 mM EDTA, 0.02% Tween 20, pH 8.0.
复融 Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景介绍

Human Interleukin-36α (IL-36α) is a secreted Cytokine that belongs to the Interleukin 1 Cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this Cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding Receptor which upon ligation, recruits a second Receptor as a signaling component, forming an active heterodimeric Receptor complex.
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RF/6A (猴脉络膜-视网膜内皮细胞)

  • 产品概述
  • 参考文献
  • 相关产品推荐

产品概述
产品信息
名称 RF/6A (猴脉络膜-视网膜内皮细胞)
背景描述 RF/6A细胞在早期自发转化并已传代540次以上;电镜下观察到Weibel-Palade颗粒。高代次的RF/6A细胞用作滋养层以增强贴壁,转接能力和供养眼色素层黑素细胞。
种属
年龄(性别) 胚胎
组织来源 脉络膜;视网膜;内皮细胞;正常
生长特性 贴壁细胞
细胞形态 上皮细胞样
细胞类型 自发永生化细胞
生物安全等级 1
生长培养基 MEM(ATCC改良)+10% FBS+1% P/S
推荐传代比例 1:2-1:4
推荐换液频率 2~3次/周
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养条件 气相:空气,95%;CO2,5% 温度:37℃
基因表达情况 factor VIII; fibronectin
保藏机构 ATCC; CRL-1780

参考文献
文献列表
  • Adiponectin inhibits high glucose‐induced angiogenesis via inhibiting autophagy in RF/6A cells(2019/04/14)

    作者:Rong Li, Junhui Du, Yang Yao

    期刊:Journal Of Cellular Physiology

    DOI:10.1002/jcp.28659

    影响因子 :6.513

    引用产品:CL-0194

  • Autophagy is required for the promoting effect of angiogenic factor with G patch domain and forkhead-associated domain 1 (AGGF1) in retinal angiogenesis(2021/07/31)

    作者:Rong Li, Guomin Yao, Lingxiao Zhou

    期刊:Microvascular Research

    DOI:10.1016/j.mvr.2021.104230

    影响因子 :3.750

    引用产品:CL-0194

  • Angiogenic factor with G patch and FHA domains 1 protects retinal vascular endothelial cells under hyperoxia by inhibiting autophagy(2020/7/7 )

    作者:Guomin Yao, Rong Li, Junhui Du

    期刊:J Biochem Mol Toxicol

    DOI:10.1002/jbt.22572

    影响因子 :3.6420

    引用产品:CL-0194

  • Angiogenic factor with G patch and FHA domains 1 protects retinal vascular endothelial cells under hyperoxia by inhibiting autophagy(2020/07/07)

    作者:Guomin Yao, Rong Li

    期刊:Journal of Biochemical and Molecular Toxicology

    DOI:10.1002/jbt.22572

    影响因子 :3.568

    引用产品:CL-0194

  • 点击展开收起列表

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RF/6A细胞专用培养基


RF/6A细胞专用培养基

货号:CM-0194

价格:¥450¥¥

促销价:¥350

规格:

125mL×4

  • 125mL×4

  • 产品概述
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产品概述

产品简介

RF/6A细胞专用培养基由普诺赛技术团队精心优化,经过长期测试,本产品可保持RF/6A细胞最佳的生长状态。本产品中已包含RF/6A细胞生长所需的各种成分,无需添加任何成分,可直接用于RF/6A细胞的体外培养。本产品仅供进一步科研使用,不得用于诊断、治疗、临床、家庭及其它用途。

产品说明

产品形态 液体
产品浓度
产品规格 125mL×4
培养基成分 MEM(ATCC改良)(PM150467)+10% FBS(164210-50)+1% P/S(PB180120)
细菌检测 阴性
真菌检测 阴性
支原体检测 阴性
细胞生长实验 细胞生长良好,形态正常
内毒素含量(EU/mL) ≤3
储存条件 2℃-8℃,避光储存
运输条件 冰袋冷藏运输
有效期 3个月

注意事项

1、使用产品时应注意无菌操作,避免污染。 2、本产品含有血清和双抗,如无特别需要不用额外再添加血清和双抗,可以直接使用。 3、为保持本产品的最佳使用效果,不宜将其长时间放置于室温或较高的温度环境中。 4、所有产品请于保质期内使用,超出保质期,必须放弃使用。 5、本产品只供进一步科研使用,不可应用于临床等其他方面。

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