Calcofluor White Stain 卡尔科弗卢尔荧光增白剂染液

Calcofluor White Stain 卡尔科弗卢尔荧光增白剂染液

货号: MM1011-100ML 品牌: Jinpan 标签: 免疫学

描述

卡尔科弗卢尔荧光增白剂  Calcofluor White Stain

 — —酵母、真菌、寄生生物荧光染料

  基本描述:

卡尔科弗卢尔荧光增白剂(Calcofluor White Stain)一种用于快速检测酵母、真菌和寄生生物的荧光染料。

 

作用机制(阐述):

卡尔科弗卢尔荧光增白剂(Calcofluor White Stain)是一种与真菌和其他生物体细胞壁中的纤维素和壳多糖结合的非特异性荧光染料,卡尔科弗卢尔荧光增白剂染色是一种快速检测方法,可检测许多酵母菌和病原性真菌比如微孢子虫(Microsporidium)、棘阿米巴(Acanthamoeba)、肺囊虫(Pneumocystis)、纳氏虫(Naegleria)和阿米巴(Balamuthia)物种。

伊文斯兰(Evans blue)在该染料中用作复染剂,当使用蓝色光激发(并非UV)检测可降低组织和血细胞内的背景荧光信号。300-440nm的光谱范围(Emax=433nm0.1M磷酸盐pH 7.0;纤维素)可用作发射光谱,激发波长约在355nm。

 

产品组分:

卡尔科弗卢尔白M2R(Calcofluor White M2R ) 1 g/L

伊文斯兰(Evans blue)  0.5 g/L

 

染色步骤:

  1. 将待检样本置于一干净载玻片上;
  2. 滴一滴卡尔科弗卢尔荧光增白剂(Calcofluor White Stain)和一滴10% 氢氧化钾;

  3. 将盖玻片置于样本上,并静置染色1min;

  4. 使用x100 to x400放大倍数紫外光下观察。

 

染色结果(阐述):

真菌或寄生生物呈亮绿到蓝色荧光,而其他材料呈红-橙色荧光。若染色组织样本会有非特异性反应。对于这些样本可能会看到黄绿色背景荧光,但真菌和寄生结构荧光强度更明亮。

Fungal or parasitic organisms appear fluorescent bright green to blue, while other material is reddish-orange fluorescent. Non-specific reactions may occur when tissues samples are used. May a yellowish-green background. fluorescence is observed with such specimens but fungal and parasitic structures appears with much more intense. Attention cotton fibers will fluoresce strongly and must therefore be differentiated from fungal hyphae. As well amebic cysts are fluorescent but trophozites will not stain or fluoresce, Background fluorescence can be diminished by examining under blue light or by using different filter combinations (emission and excitation filters). One drop of 10% potassium hydroxide solution can be added for better visualization of fungal elements.

 

订购信息:即用型的染料,含Calcofluor White M2R和Evans blue,操作极其简单。进口原料,品质保证,现货销售。

 品牌  名称  货号  规格  价格(元)
 JP      Calcofluor White Stain卡尔科弗卢尔荧 光增白剂  MM1011-100ML   100ml  648.00

另提供荧光增白剂 Calcofluor White M2R(Fluorescent Brightener 28,CAS NO. 4404-43-7 ) 578/1g,冻干粉形式,更加经济实惠,欢迎来电咨询。电话:021-50837765.

 

注意事项

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为了您的安全和健康,请穿实验服并戴一次性手套操作。

 

 

规格信息

品牌:

Jinpan

CAS:

4404-43-7

规格:

100ml

货期:

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