Minimal SD Base SD基础培养基
货号: JP6312-267G 品牌: Jinpan 标签: 生化试剂
描述
Minimal SD Base SD基础培养基
产品标签
YPD Medium(YEPD Medium);YPDA Medium;YNB;Minimal SD Base;Dropout (DO) Supplements 省却成分添加剂;
产品信息
产品名称 | 产品编号 | 规格 | 价格(元) |
Minimal SD Agar Base SD琼脂基础培养基 | JP6311-467G | 467g | 1428 |
Minimal SD Base SD基础培养基 | JP6312-267G | 267g | 1048 |
产品描述
Minimal SD Base,即Minimal Synthetical Defined Medium,是在无氨基酸酵母氮源基础(YNB)上添加单一碳源如葡萄糖、半乳糖、棉子糖和琥珀酸盐或多碳源搭配而合成的培养基基础。常常与省却成分添加剂(Dropout (DO) Supplements)联合使用,以确认酵母菌的营养缺陷表型和筛选酵母转化子。
保存与运输方法
保存:室温干燥保存,至少2年稳定。
运输:室温运输。
培养基配制
- 加入相应量的Minimal SD Base和DO Supplements【见下表1】到1L去离子水,搅拌使其溶解。
- 重要:测试培养基pH是否为5.8,若有必要用弱酸或弱碱调整pH。
- 121℃高压灭菌15min。
- 柔光下4℃保存高压灭菌后的液体培养基。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。
相关产品
货号 | 名称 | 规格 |
JP6301-350G | YPD Agar Medium YPD琼脂培养基 | 250g |
JP6302-250G | YPD Medium YPD液体培养基 | 250g |
JP6303-350G | YPDA Agar Medium YPDA琼脂培养基 | 350g |
JP6304-250G | YPDA Medium YPDA液体培养基 | 250g |
JP6305-100G | YNB Without Amino Acids酵母氮源基础(无氨基酸) | 100g |
JP6305-500G | YNB Without Amino Acids酵母氮源基础(无氨基酸) | 500g |
JP6311-467G | Minimal SD Agar Base SD琼脂基础培养基 | 467g |
JP6312-267G | Minimal SD Base SD基础培养基 | 267g |
JP6313-270G | Minimal SD Agar Base Gal/Raf SD琼脂基础培养基(含半乳糖/棉子糖) | 270g |
JP6314-185G | Minimal SD Base Gal/Raf SD基础培养基(含半乳糖/棉子糖) | 185g |
表1 酵母培养基配制浓度表
培养基类型 | 名称 | 货号 | 终浓度(g/L) |
Rich Media
富集培养基 |
YPD Agar Medium | JP6301-350G | 70 |
YPD Medium | JP6302-250G | 50 | |
YPDA Agar Medium | JP6303-350G | 70 | |
YPDA Medium | JP6304-250G | 50 | |
Minimal Media
基本培养基 |
Minimal SD Agar Base | JP6311-467G | 46.7 |
Minimal SD Base | JP6312-267G | 26.7 | |
Minimal SD Agar Base Gal/Raf | JP6313-270G | 54 | |
Minimal SD Base Gal/Raf | JP6314-185G | 37 | |
Single Dropout Supplements (SDO)
单一省却成分添加剂 |
DO Supplement -His | JP6316-10G | 1.29 |
DO Supplement -Leu | JP6317-10G | 1.29 | |
DO Supplement -Trp | JP6318-10G | 1.29 | |
DO Supplement -Ura | JP6319-10G | 1.29 | |
Double Dropout Supplements (DDO)
双省却成分添加剂 |
DO Supplement -His/-Leu | JP6320-10G | 1.19 |
DO Supplement -His/-Ura | JP6321-10G | 1.27 | |
DO Supplement -Leu/-Trp | JP6322-10G | 1.19 | |
DO Supplement -Met/-Trp | JP6323-10G | 1.27 | |
DO Supplement -Trp/-Ura | JP6324-10G | 1.27 | |
DO Supplement -Trp/-His | JP6325-10G | 1.27 | |
DO Supplement -Ura/-Leu | JP6326-10G | 1.27 | |
Triple Dropout Supplements (TDO)
三省却成分添加剂 |
DO Supplement -His/-Leu/-Trp | JP6331-10G | 1.17 |
DO Supplement -His/-Leu/-Ura | JP6332-10G | 1.17 | |
DO Supplement -His/-Trp/-Ura | JP6333-10G | 1.25 | |
DO Supplement -Leu/-Met/-Trp | JP6334-10G | 1.17 | |
DO Supplement -Leu/-Trp/-Ura | JP6335-10G | 1.17 | |
Quadruple Dropout Supplements (QDO)
四省却成分添加剂 |
DO Supplement -Ade/-His/-Leu/-Trp | JP6341-10G | 1.15 |
DO Supplement -His/-Leu/-Met/-Trp | JP6342-10G | 1.15 | |
DO Supplement -His/-Leu/-Trp/-Ura | JP6343-10G | 1.15 |
— — Written/Edited by V. Shallan【版权归集奇生物/JP金畔所有】
规格信息
品牌: |
Jinpan |
---|---|
CAS: |
N/A |
规格: |
267g |
货期: |
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